Journal: Neurobiology of aging

Article Title: Life-long Overexpression of S100? in Down's Syndrome: Implications for Alzheimer Pathogenesis

doi:

Figure Lengend Snippet: Photomicrographs showing β-APP+ neurons in neocortex of young (Y) and adult (A) Down’s (DS) and control (C) patients (top four panels). Lower two panels show Tau2+ neurons in adult Down’s (A-DS) but not control (A-C) patients. Bars = 15 µm.

Article Snippet: Paraffin blocks were serially sectioned at a thickness of 10 µm, and immunoreaction of rabbit-anti-bovine S100β (a gift from Dr. Linda Van Eldik ( 21 )) and mouse-anti-human β-APP (clone 22C11, Boehringer-Mannheim) antibodies was performed on glass slide-mounted tissue sections as previously described ( 13 ).

Techniques:

Journal: Neurobiology of aging

Article Title: Life-long Overexpression of S100? in Down's Syndrome: Implications for Alzheimer Pathogenesis

doi:

Figure Lengend Snippet: Numerical density of β-APP+ neurons (A) and of Tau2+ neurons (B) in neocortex of Down’s and control patients. For β-APP+ neurons, the mean values were significantly different for young (* p < 0.02), and for adult (** p < 0.0001), but not for fetal age ranges. For Tau2+ neurons the mean values were significantly different for adults (** p < 0.0001).

Article Snippet: Paraffin blocks were serially sectioned at a thickness of 10 µm, and immunoreaction of rabbit-anti-bovine S100β (a gift from Dr. Linda Van Eldik ( 21 )) and mouse-anti-human β-APP (clone 22C11, Boehringer-Mannheim) antibodies was performed on glass slide-mounted tissue sections as previously described ( 13 ).

Techniques:

Journal: Neurobiology of aging

Article Title: Life-long Overexpression of S100? in Down's Syndrome: Implications for Alzheimer Pathogenesis

doi:

Figure Lengend Snippet: Dual plots of the numerical density of β-APP+ neurons vs. numerical density of S100β+ astrocytes for young and adult Down’s (●) and control (○) patients show a separation of the two patient populations. A regression line is shown for the Down’s syndrome values only. Values were significantly correlated for Down’s (r = 0.75, p < 0.05) but not for control patients.

Article Snippet: Paraffin blocks were serially sectioned at a thickness of 10 µm, and immunoreaction of rabbit-anti-bovine S100β (a gift from Dr. Linda Van Eldik ( 21 )) and mouse-anti-human β-APP (clone 22C11, Boehringer-Mannheim) antibodies was performed on glass slide-mounted tissue sections as previously described ( 13 ).

Techniques:

Journal:

Article Title: Involvement of notch signaling pathway in amyloid precursor protein induced glial differentiation

doi: 10.1016/j.ejphar.2010.09.015

Figure Lengend Snippet: (A) Representative fluorescent immunohistochemistry of wild type and APP23 mice brain 4–6 weeks after HNPCs transplantation. More human GFAP immunopositive cells were observed in the cortex of APP23 (ii) compare to wild type mice (i) at 8 months old. Human GFAP immnopositive cells showing active gliogenesis morphology were detected around plaque-like formations in the cortex of 12 month old APP23 transgenic mice (iii). Green and red stainings represent immunoreactivity for GFAP and Aβ recognized by 4G8, respectively. All the nuclei were counter stained by DAPI. (B) Western blot analysis of protein expression of APP, GFAP, and NICD in the brain of 8 months old WT and APP23 transgenic mice. Anti-APP and anti-NICD antibodies were used to recognize APP and NICD, respectively. One-factor ANOVA followed by post hoc analysis (Student-Newman-Keuls) was used to analyze the differences between experimental groups and control groups (*: p<0.05, **: p<0.01).

Article Snippet: Next, the samples were incubated overnight at 4 C (up to 12 h) with the following primary antibodies: mouse anti human Aβ antibody, 4G8 (1:50, Senetek); mouse IgG1 6E10, (1:50, Senetek); mouse IgG anti-Alzheimer Precursor Protein A4, 22C11 (1:100, Chemicon); mouse IgG2b anti-human βIII-Tubulin, clone SDL3D10 (1:1000, Sigma) and goat anti human-glial filbrillary protein, GFAP (N-terminal human affinity purified, 1:400, Research Diagnostics Inc., Flander, NJ).

Techniques: Immunohistochemistry, Transplantation Assay, Transgenic Assay, Staining, Western Blot, Expressing